Composite

Part:BBa_K2680123

Designed by: Ethan M Jones   Group: iGEM18_William_and_Mary   (2018-10-06)
Revision as of 19:43, 13 October 2018 by Jaurban (Talk | contribs)


3G pTlpA

3G-compatible pTlpA promoter BBa_K2500003. Repressed by TlpA, a heat-sensitive protein which begins to deactivate at 37C. pTlpA also transcribes 500 fold weaker in the reverse direction. Flanked by prefix sticky end A and suffix sticky end B.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 41
    Illegal BsaI.rc site found at 109


[edit]
Categories
Parameters
None