Part:BBa_K2680524

pLac/ara-1

Repressed by LacI and activated by arabinose/AraC. Intended for use in oscillator creation. Sequence originally from Lutz and Bujard 1997; later used for the creation of oscillator from Stricker et al 2008.^1,2 Note that the upstream LacO site is required for functional repression via LacI (tetrameter) [See Stricker et al 2008 supplement].

References

¹Lutz, R., & Bujard, H. (1997). Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Research, 25(6), 1203–1210.

²Stricker, J., Cookson, S., Bennett, M. R., Mather, W. H., Tsimring, L. S., & Hasty, J. (2008). A fast, robust and tunable synthetic gene oscillator. Nature, 456(7221), 516–519. https://doi.org/10.1038/nature07389

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 425
  Illegal XhoI site found at 392
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]