Composite
Part:BBa_K2683019:Design
Designed by: Kristi Turton Group: iGEM18_Lethbridge (2018-10-10)
Lysine tRNA Synthetase (LysRS) in pSB1C3
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1459
Illegal NgoMIV site found at 1531 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 733
Design Notes
The subparts and coding sequence of this part is codon optimized for use in E.coli .
Source
https://parts.igem.org/Part:BBa_K2443012
References
[1] Friest, W and Gauss, D. (1995) Lysyl-tRNA Synthetase. Biology Chemistry Hoppe Seyler. 377, 399-402