RNA

Part:BBa_K2725016:Experience

Designed by: Eva Steele   Group: iGEM18_Edinburgh_UG   (2018-10-11)
Revision as of 22:07, 17 October 2018 by Evasteele (Talk | contribs)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K2725016

Part of the semantic containment system for preventing horizontal gene transfer. This serine amber suppressor tRNA expressed under J23108 allows expression of a protein coding sequence with 5 serine codons substituted for amber stop codons (TAG).

<figure class="figure">

             <img src="T--Edinburgh_UG--400.png" width=“10%" height="10%" align="left">
  <figcaption class="figure-caption">Figure 2. Growth curves of 8 Top10 E.coli double transformants in 400 μg/mL kanamycin. These growth curves show the extent of kanamycin resistance conferred by each part combination. </figcaption>

</figure>

User Reviews

UNIQ41e714ebc12d00dd-partinfo-00000000-QINU UNIQ41e714ebc12d00dd-partinfo-00000001-QINU