Part:BBa_K2623023

Bacterial outer membrane protein A (OmpA) fused with SpyTag and GFP at its N-termini

SUMMARY

It’s another form of the BBa_K2623021 designed to target our archaeal ribosomal protein protein L7Ae and then siRNA into outer-membrane vesicles (OMVs). The only difference is that we inserted SpyTag to C-termini of OmpA protein(instead of the N-termi in BBa_K2623021). As mentioned in BBa_K2623021, SpyTag was constructed in order to anchore L7Ae to outer membrane through the bioconjugation of the SpyTag/SpyCatcher system. Through the fluorescence intensity of GFP, we can compare and evaluate expression efficiency of SpyTag between the BBa_K2623021 and this part. Then the better one would be uesd to construct our final gene circuit.

Other information

You can click the website (http://2018.igem.org/Team:XMU-China/Results) to see the results of comparison of two reporters (BBa_K2623021 and BBa_K2623023)<a href="http://2013.igem.org/Team:TU-Munich/Results/Software">documentation</a> Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 65
  
• 1000
  COMPATIBLE WITH RFC[1000]