Part:BBa_K2889000:Experience

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K2889000

1.cell migration

1.1 Cell migration is a central process in the development and maintenance of tumor. We cloned the full length of IL7-AS-S2 into PCDNA3.1 and transfected the plasmid to 786-O cells.Through in vitro scratch wound healing assay, overexpression of IL7-AS-S2 promoted cell migration of 786-O cells.Overexpression of IL7-AS-S2 increased the migration of 786-O cells (Fig. 1 and 2). These results suggested that IL7-AS-S2 contain key structural domains.

1.2 In order to investigate whether cell migration induced by IL7-AS-S2 depends on the amount of IL7-AS-S2 transfection. We transfected the different concentration of IL7-AS-S2 () into 786-0 cells.(Fig. 3 and 4)

2.IL7-AS-S2 contains a complicated secondary structure

In silico prediction of lncRNA secondary structure is another useful method to assign putative functions to non-coding transcripts, based upon the widely held assumption that highly folded structures impart functionality through binding interactions with proteins/nucleotides. Characterization of IL7-AS, IL7-AS-S1 and IL7-AS-S2 using RNAfold minimum free energy estimations predicted a highly folded secondary structure with several hairpin loops, which imply interact with protein (Fig. 5).

The optimal secondary structure with a minimum free energy. The secondary structures of IL7-AS-S2 were predicted by the RNAfold webserver (http://rna.tbi.univie.ac.at/cgi-bin/RNAfold.cgi). The structure is colored according to base-pairing probabilities. For unpaired regions, the color denotes the probability of being unpaired.

User Reviews

UNIQ04b3bdb4a394f611-partinfo-00000000-QINU UNIQ04b3bdb4a394f611-partinfo-00000001-QINU