Coding

Part:BBa_K2665014

Designed by: You Tong   Group: iGEM18_Kyoto   (2018-10-08)
Revision as of 03:29, 11 October 2018 by Nanbu (Talk | contribs)


TDH3 promoter->SseNHX1->6xHis->CYC terminator

SseNHX1

Usage and Biology

SseNHX1 is the vacuolar Na(+)/H(+) antiporter. This antiporter enables to survive high osmotic pressure by sequestering excessive cytoplasmic Na(+) into vacuole. 

The gene, SseNHX1 is created by DNA family shuffling;  Two homologous Na(+)/H(+) antiporters from halophytes Salicornia europaea (SeNHX1) and Suaeda salsa (SsNHX1) were shuffled.

By reference, SseNHX1 enables higher salt tolerance and sequester Na(+) than the two protein, SeNHX1 and SsNHX1.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2379
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None