Coding

Part:BBa_K2692000:Design

Designed by: Kristi Turton   Group: iGEM18_Lethbridge_HS   (2018-10-08)
Revision as of 03:19, 11 October 2018 by Kristiturton (Talk | contribs) (→‎Design Notes)


CutA metal binding protein from E.coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The hexahistidine tag has the ability to also bind to metals (this is why is is able to bind to a nickle column). To gain accurate results, the his tag would have to be removed to ensure that the cutA protein is binding the copper.

Source

The part (in plasmid) was a gift from the Weiden lab at the University of Lethbridge and is originally a plasmid from the ASKA database.

References

[1] Zhao, Y., Wang, Y., Hu, J., Zhang, X., and Zhang, Y. (2012) CutA divalent cation tolerance homolog (Escherichia coli) (CUTA) regulates beta-cleavage of beta-amyloid precursor protein (APP) through interacting with beta-site APP cleaving protein 1 (BACE1). Journal of Biological Chemistry 287, 11141-11150