Coding

Part:BBa_K2617017

Designed by: Changyu Li   Group: iGEM18_UESTC-China   (2018-09-30)
Revision as of 03:05, 11 October 2018 by Yet (Talk | contribs)


J23100-RBS-pelB-5D

A combination system of promoter, ribosome binding site and signal peptide for enhancing protein expression(pelB)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 115
  • 1000
    COMPATIBLE WITH RFC[1000]

Characterization

We have improved the previous part BBa_J23100 by adding RBS and pelB-5D to make it can be used for extracelluar expression. The combination of promoter, RBS and signal peptide makes it convenient to use as a biobrick and gives it the function of extracellular expression.



[edit]
Categories
//chassis/prokaryote/ecoli
Parameters
None