Coding
Part:BBa_K2692001:Design
Designed by: Kristi Turton Group: iGEM18_Lethbridge_HS (2018-10-08)
Major T4 Capsid Protein gp23
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 9
Illegal PstI site found at 342
Illegal PstI site found at 1524 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 9
Illegal PstI site found at 342
Illegal PstI site found at 1524
Illegal NotI site found at 15 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 9
Illegal BamHI site found at 1069 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 9
Illegal PstI site found at 342
Illegal PstI site found at 1524 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 9
Illegal XbaI site found at 24
Illegal PstI site found at 342
Illegal PstI site found at 1524 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1118
Design Notes
The addition of a his tag for purification purposes
Source
The part was synthesized as a g-block (linear Piece of DNA) through IDT.
References
[1] Van der Vies, S., Gatenby, A and Georopoulos, C. (1994) Bacteriphage T4 encodes a co-chaperonin that can substitute for Escherichia coli <i/> GroES in protein folding. Nature. 368, 654-656