Composite

Part:BBa_K2859000

Designed by: Kyuhee Jo   Group: iGEM18_HAFS   (2018-10-10)
Revision as of 22:22, 17 October 2018 by Kyuhee0622 (Talk | contribs)


Insulin secretion (Strong)

This part secretes Winsulin, single chain insulin, associated with penetratin and FLAG tag that allows easy detection with western blot. The coding sequence is regulated by promoter J23100.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



<img src="https://bit.ly/2P5vTZH">

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Categories
Parameters
None