Part:BBa_K2617000

Xyn10D-Fae1A：Bifunctional xylanase/ferulic acid esterase

Xyn1D-fae1A cuts off the crosslinking between polysaccharides and lignin in cell wall and degrade xylose hemicellulose. This part is responsible for pretreatment of straw.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 534
  Illegal AgeI site found at 381
  Illegal AgeI site found at 628
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 1236
  

Characterization

Molecular weight

This halohydrin dehalogenase gene codes for a protein of 726 amino acids with a molecular mass of 82.3 kDa.

Enzyme activity

Fig. 1 Standard Curve of Xylose. Data were measured in phosphate buffer solution at pH 6.0 and 40℃.

Fig. 2 Xylanase Activity on Xylan. Data were measured inphosphate buffer solution at pH 6.0 and 40℃.

Fig. 3 Enzyme Activity of Ferulic acid esterase. Data were measured in phosphate buffer solution at pH 6.4 and 40℃.