Part:BBa_K2610041

pGapA-AmilCP

Promoter GapA is naturally occurring constitutive promoter in Escherichia coli.

Usage and Biology

We, iGEM Leiden 2018, have combined this naturally occurring constitutive promoter ([parts.igem.org/Part:BBa_K2610040 BBa_K2610040]) from Escherichia coli with the blue chromoprotein AmilCP ([parts.igem.org/Part:BBa_K592009 BBa_K592009]) in order for us to compare it to our novel stress promoter+chromoprotein constructs.

Contrary to our stress promoters, this promoter has a constitutive high basal expression level. In contrast to many synthetic promoters, this novel promoter provides an indication of high levels of natural expression. Therefore, this promoter can be used as a future positive control of reporter production - such as GFP - in a natural situation. Moreover, GapA being a natural occurring promoter means that there are still possibilities for optimization through radiation. This way variations of this promoter can provide access to an even wider spectrum of colors.

Figure 1. Constitutively expressed pGapA-AmilCP compared to non-transformed DH5a and pGapA-spis-Pink (pink chromoprotein)

References

Munjal, N., Jawed, K., Wajid, S., & Yazdani, S. S. (2015). A constitutive expression system for cellulase secretion in Escherichia coli and its use in bioethanol production. PloS one, 10(3), e0119917.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 140