Reporter

Part:BBa_K2610041

Designed by: Charlotte de Ceuninck van Capelle, Paula Regal   Group: iGEM18_Leiden   (2018-10-10)
Revision as of 11:33, 10 October 2018 by Chardeceuninck (Talk | contribs) (→‎Usage and Biology)


pGapA-AmilCP

Promoter GapA is naturally occurring constitutive promoter in Escherichia coli.

Usage and Biology

We, iGEM Leiden 2018, have combined this naturally occurring constitutive promoter from Escherichia coli with the blue chromoprotein AmilCP (BBa_K592009) in order for us to compare it to our novel stress promoter+chromoprotein constructs. GapA is known for its high constitutive expression, as is demonstrated in Figure 1.

Moreover, GapA being a natural occurring promoter there are still possibilities for optimization through radiation. This way variations of this promoter can provide access to an even wider spectrum of colors.

T--Leiden--gapaamilcp.jpeg

Figure 1. Constitutively expressed AmilCP

References

Munjal, N., Jawed, K., Wajid, S., & Yazdani, S. S. (2015). A constitutive expression system for cellulase secretion in Escherichia coli and its use in bioethanol production. PloS one, 10(3), e0119917.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 140


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