Coding

Part:BBa_K2872019

Designed by: Dina Altarawneh   Group: iGEM18_CMUQ   (2018-10-10)
Revision as of 11:33, 10 October 2018 by Dtarawne (Talk | contribs)


Cas12a protein

This part was synthesized using Cas12a DNA coding sequence from pY016 (pcDNA3.1-hLbCpf1) plasmid (Addgene plasmid # 69988) [1]

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1198
    Illegal PstI site found at 1696
    Illegal PstI site found at 3403
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1198
    Illegal PstI site found at 1696
    Illegal PstI site found at 3403
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 938
    Illegal BglII site found at 1271
    Illegal BglII site found at 1841
    Illegal BglII site found at 2108
    Illegal BglII site found at 2729
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1198
    Illegal PstI site found at 1696
    Illegal PstI site found at 3403
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1198
    Illegal PstI site found at 1696
    Illegal PstI site found at 3403
    Illegal NgoMIV site found at 1000
    Illegal NgoMIV site found at 1999
    Illegal NgoMIV site found at 2701
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1329
    Illegal SapI.rc site found at 1389


[edit]
Categories
Parameters
None