Reporter

Part:BBa_K2610035

Designed by: Charlotte de Ceuninck van Capelle, Maaike de Jong   Group: iGEM18_Leiden   (2018-10-10)
Revision as of 12:01, 11 October 2018 by Chardeceuninck (Talk | contribs)


pSoxS-GFP-GFP-pSoxS-GFP-GFP

This composite part features twice the regulatory part promoter SoxS (BBa_K2610030) and two times the fluorescent protein GFP (BBa_E0040). It can be used to visualize upregulation of SoxS as a result of superoxide stress.

Regulatory protein SoxS is involved in the superoxide pathway in Escherichia coli. It acts a superactivator of downstream stress genes.

Usage and Biology

We, iGEM Leiden 2018, have designed this composite part as part of our project Fifty Shades of Stress. This reporter part allowed us to detect stress-induced changes in SoxS transcription. We have created this part in order to amplify the fluorescent signal measured after pSoxS-GFP activation.

As was demonstrated in Figure 1 pSoxS-GFP-GFP-pSoxS-GFP-GFP responds to nalidixic acid in a dose-dependent response curve.

T--Leiden--amplification4.png

Figure 1. Dose-response curve as a result of treatment with nalidixic acid.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 947
    Illegal BsaI.rc site found at 1673
    Illegal BsaI.rc site found at 2704
    Illegal BsaI.rc site found at 3430


[edit]
Categories
Parameters
None