Coding

Part:BBa_K2685004:Design

Designed by: LIN, WENXIN   Group: iGEM18_TUST_China   (2018-10-06)
Revision as of 07:07, 10 October 2018 by PandaLIN (Talk | contribs)

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TetA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1043
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 897
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 343
    Illegal NgoMIV site found at 503
    Illegal NgoMIV site found at 871
  • 1000
    COMPATIBLE WITH RFC[1000]

TetA - Tetracycline resistance protein, class C - Escherichia coli - tetA gene & protein Resistance to tetracycline by an active tetracycline efflux. This is an energy-dependent process that decreases the accumulation of the antibiotic in whole cells. This protein functions as a metal-tetracycline/H(+) antiporter


Design Notes

pump out the isomerical tetracycline


Source

From the PCR methods

References