Regulatory

Part:BBa_K2596006

Designed by: Priya Aggarwal, Manvi Shah   Group: iGEM18_Stony_Brook   (2018-10-09)
Revision as of 04:55, 16 October 2018 by Kledalla (Talk | contribs)


Pcpc560 from Synechocystis sp. PCC 6803

This is a strong constitutive promoter from cyanobacteria Synechocystis sp. PCC 6803

Usage and Biology

Pcpc-560 is a unique form of the Pcpc promoter native to Synechocystis sp. 6803. It includes the two cpc promoters (as described for Pcpc) and 14 Transcription Factor Binding Sites, allowing it to be an incredibly strong promoter. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

For the BioBrick, we kept the full 560 bp of Pcpc-560, and did not include an upstream region. The full cpc-560 promoter ends immediately before the ATG start codon in its native form.


[edit]
Categories
//chassis/prokaryote/cyanobacterium
//promoter
Parameters
chassisSynechococcus elongatus PCC 7942
device_typeConstitutive Promoter