Composite

Part:BBa_K2843002

Designed by: Camille Lorie Michael de Figueiredo   Group: iGEM18_East_Chapel_Hill   (2018-10-09)
Revision as of 21:32, 17 October 2018 by Camillelorie (Talk | contribs)

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Fluoride RBS Mutant

This is BBa_K2843000 operon with the "Fluoride Binding Mutant", which is a mutant of the fluoride riboswitch. This serves as a controlled experiment because if there is growth of delta-CRCB Ecoli on the chloramphenicol plates than this indicates that growth of delta-CRCB is not entirely dependent only on the presence of fluoride.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 47
    Illegal NheI site found at 70
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 148
    Illegal XhoI site found at 189
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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