Part:BBa_K2609000

Murine JE/MCP-1

Member of CC chemokine family that uses the CCR2 receptor for chemo-attracting monocytes to the site of release (also called monocyte chemoattractant protein - 1). This coding sequence lacks the heavily glycosylated C-terminus present in the wild-type protein and has been shown to have a increased chemotactic potency^[1][2]. The protein can be expressed recombinantly in a prokaryotic system because of its lack of glycosylation and post-translational modifications.

Biology

Mcp-1 belongs to the CC family of chemokines and is recognised by the CCR2 receptors found on murine monocytes. Due to its inherent homology to human CCL2, it acts as a effective chemokine for human moncytes as well. The N-term region of the protein has been implicated in receptor binding and any fusion to this terminal might result in decreased activity. In both mice and humans, the chemokine is produced by a wide variety of cell types including endothelial,fibroblasts, epithelial, smooth muscle, mesangial, astrocytic, monocytic, and microglial cells. Apart from monocytes, CCL2 has also been shown to chemoattract NK cells and T cells with comparatively lesser efficacy.^[2]

Usage

For their Phage Assisted Immune Recruitment (PAIR) system, the 2018 IISc-Bangalore iGEM team used the mcp-1 CDS fused with both N-term and C-term secretion tags (PelB and HlyA respectively) for recombination into a phage genome. This engineered phage will recruit phagocytic monocytes to the site of infection and prevent release of bacterial toxins directly into the body.

Characterization

Purification with BBa_K2609007

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

[1] Yao, Yao, and Stella E. Tsirka. "Mouse monocyte chemoattractant protein 1 (MCP1) functions as a monomer." The international journal of biochemistry & cell biology 55 (2014): 51-59.

[2] Deshmane, Satish L., et al. "Monocyte chemoattractant protein-1 (MCP-1): an overview." Journal of interferon & cytokine research 29.6 (2009): 313-326.