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DNA
Part:BBa_K2614000:Design
Designed by: Nicole Banholzer, Rick Seifert Group: iGEM18_JMU_Wuerzburg (2018-10-09)
template as positive control for a qPCR that detects Plasmodium
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We amplifiy the template by a PCR and digest it with EcoRI-HF an PstI-HF. Subsequently we digest the pSB1C3 plasmid with EcoRI-HF and PstI-HF and ligate the digested Plasmid with our digested template.
Source
As template we used a short, synthetic, non toxic, non pathogene oligonucleotide.