Part:BBa_K2862008
pSoxS Mutant 1
pSoxS is one half of the pSoxR/pSoxS bidirectional promoter. Transcription downstream of pSoxS is activated in response to oxidation of the SoxR transcription factor, either directly by redox-cycling drugs or by oxidative stress. It is therefore inducible by various redox-cycling drugs, toxins, antibiotics, heavy metals, hydrogen peroxide and nitric oxide: providing various applications in the development of environmental and therapeutic devices. By coupling oxidation of redox-cycling species to an electrode, the 2018 Imperial College London iGEM team (PixCell) used pSoxS to build electrogenetic devices where electrical inputs modulated gene expression.
This pSoxS promoter was taken from E. coli with mutations being introduced at the -35/-10 site in order to introduce more common nucleotides to increase promoter activity. The promoter was also engineered to be unidirectional by knocking out activity of the pSoxR portion. It was designed with an upstream terminator to remove the need for these to be added as parts in large assemblies. It also contains a downstream ribozyme to reduce context-dependency. It forms part of the PixCell library of electrogenetic and redox-sensing parts.
This part is compatible for BioBrick, BASIC and Golden Gate assembly.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 49
Illegal BsaI.rc site found at 286
| None |