Part:BBa_I715052:Experience

Number of green fluorescent cells per 10,000 counted by the flow cytometer. The most striking result is the GFP expression of the Trp-GFP.lva plasmid in LB and in M9 media, a more than one hundred-fold difference. Also noteworthy is the lack of difference for this same plasmid for the MC4100 strain, despite the otherwise strong GFP expression in MC4100.

Flow cytometry data for JM109 cells grown in the presence (left) and absence (right) of tryptophan. In both conditions, cells without GFP plasmid do not exhibit green fluorescence (blue). Cells with GFP regulated by the trp operon regulatory sequence do not express GFP when there is tryptophan present, but do express GFP when there is no tryptophan (red).

Applications of BBa_I715052

This part is intended to terminate transcription in the presence of tryptophan at high concentrations. It is to be placed downstream of a promoter, but upstream of the regions not to be transcribed.

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