Coding
paroR

Part:BBa_K2703008:Design

Designed by: Aurelie Bouin, Victor Sayous   Group: iGEM18_Sorbonne_U_Paris   (2018-10-09)
Revision as of 10:22, 10 October 2018 by Saniya kari (Talk | contribs) (→‎Design Notes)


paromycin resistance gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

From the aphVIII gene sequence of the BioBrick BBa_K1884013 (iGEM16_SZU-China) XXX’s restriction site was removed ( G551C) by PCR point mutation. We cloned aphVIII by PCR amplification in B4-B5 position of the MoClo standard2 to use it in our retrotransposon design

Source

The part comes from the Chlamydomonas MoClo tookit (Crozet et al., 2018). We received the part from the lab of the authors

References