DNA

Part:BBa_K2889003:Experience

Designed by: Rui Zhou   Group: iGEM18_Worldshaper-Wuhan   (2018-10-04)
Revision as of 04:43, 9 October 2018 by Ruizhou (Talk | contribs) (→‎Applications of BBa_K2889003)


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Applications of BBa_K2889003

Electrophoresis_of_miR-21-sponge-4s_PCR_product.jpg


First we amplified miR-21-sponge-4s using primers. After that, we purified the PCR products by PCR Purification Kit and digested them with restriction enzymes EcoR I and Pst I

Digested_the_PSB1C3_vectors_with_EcoRI_and_PstI.jpeg

We digested the PSB1C3 vectors with restriction enzymes EcoRI and PstI


MiR-21-sponge-4s_sequencing_map.jpeg

We selected the positive clones by PCR and sequencing

User Reviews

UNIQ6a3942ed12c37833-partinfo-00000000-QINU UNIQ6a3942ed12c37833-partinfo-00000001-QINU