DNA

Part:BBa_K2889003:Experience

Designed by: Rui Zhou   Group: iGEM18_Worldshaper-Wuhan   (2018-10-04)
Revision as of 04:43, 9 October 2018 by Ruizhou (Talk | contribs) (→‎Applications of BBa_K2889003)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K2889003

Electrophoresis_of_miR-21-sponge-4s_PCR_product.jpg


First we amplified miR-21-sponge-4s using primers. After that, we purified the PCR products by PCR Purification Kit and digested them with restriction enzymes EcoR I and Pst I

Digested_the_PSB1C3_vectors_with_EcoRI_and_PstI.jpeg

We digested the PSB1C3 vectors with restriction enzymes EcoRI and PstI


MiR-21-sponge-4s_sequencing_map.jpeg

We selected the positive clones by PCR and sequencing

User Reviews

UNIQ62c75eb471886f75-partinfo-00000000-QINU UNIQ62c75eb471886f75-partinfo-00000001-QINU