Coding
Part:BBa_K2665012
Designed by: You Tong Group: iGEM18_Kyoto (2018-10-08)
TDH3-ZrGPD1-6xHis-CYC
ZrGPD1 is the gene that codes a glycerol-3-phosphate dehydrogenase which controls glycerol synthesis in Zygosaccharomyces rouxii. It increases glycerol synthesis which work as compatible solute. So, it may raise osmotolerance in yeast.
For the confirmation of the mangrin function of mangrin, we spotted
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1923
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1454
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1129
Illegal BsaI site found at 1889
Illegal BsaI.rc site found at 1271
Reference
[1] Hou,Lihua Wang,Meng Wang,Cong Wang,Chunling Wang,Haiyong (2013) Analysis of salt-tolerance genes in zygosaccharomyces rouxii, Applied Biochemistry and Biotechnoloogy 1417-1425
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Categories
Parameters
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