Coding
Part:BBa_K2692002:Design
Designed by: Kristi Turton Group: iGEM18_Lethbridge_HS (2018-10-08)
Major T4 Capsid Protein gp24
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal suffix found in sequence at 1577
- 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 1578
Illegal PstI site found at 1592
Illegal NotI site found at 1585 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 753
Illegal BamHI site found at 1248 - 23INCOMPATIBLE WITH RFC[23]Illegal suffix found in sequence at 1578
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 1578
Illegal PstI site found at 1592 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The addition of a his tag was considered for purification purposes
Source
This part was synthesized as a g-block
References
[1] Olson N., Gingery, M., Eiserling, F., and Baker, T. (2001) The structure of isometric capsids of bacteriphage T4. Virology. 279,385-391