Coding
Part:BBa_K2683001:Design
Designed by: Kristi Turton Group: iGEM18_Lethbridge (2018-10-07)
Arc Protein Codon optimized for E.coli
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 531
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part is codon optimized for use in E.coli .
Source
The original sequence is derived from the Arc protein found in mouse neural cells. The DNA was amplified from the mouse genome and made biobrick compatable by using primer extension PCR and site directed mutagenesis.