Protein_Domain

Part:BBa_K2543006:Design

Designed by: CHUNG-HSUAN HSIAO   Group: iGEM18_Mingdao   (2018-10-08)
Revision as of 17:31, 8 October 2018 by 61XB38 (Talk | contribs) (→‎References)


hCD4 extracellular domain / pSB1C3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1138
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1189
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 370


Design Notes

This part follows the RFC[25] assembly rule with an AgeI site in the 3' end.


Source

This part was synthesized by Integrated DNA Technologies, Inc. (IDT) and cloned onto pSB1C3.


References

1. UniProtKB - P01730 (CD4_HUMAN)
2. Retrovirology. (2006) Association between disruption of CD4 receptor dimerization and increased human immunodeficiency virus type 1 entry 3. J Immunol. (2006) Evidence for a domain-swapped CD4 dimer as the coreceptor for binding to class II MHC. 4. J Immunol. (2006) Triggering of T cell activation via CD4 dimers. 5. J Biol Chem. (2014) Disulfide reduction in CD4 domain 1 or 2 is essential for interaction with HIV glycoprotein 120 (gp120), which impairs thioredoxin-driven CD4 dimerization.