Coding

Part:BBa_K2684001

Designed by: Yihao Zhang   Group: iGEM18_SHSBNU_China   (2018-10-08)
Revision as of 15:56, 17 October 2018 by JimLi2002 (Talk | contribs)


PelB-CotA

CotA laccase attached with PelB signal peptide sequence

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 54
    Illegal AgeI site found at 1411
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 349


Overview

PelB is a type of signal peptide linking with CotA. It can transfer CotA out of E.coli. We tested the enzyme activity of our part based on the weight of our sample. Formula: laccase activity (nmol/min/g) = ΔA /ε(ABTS mmolar extinction coefficient) / d * V (total volume of reaction) / V (volume of sample in the reaction, 0.025mL) / W (sample mass, g) * V (extracted liquid added, 1mL) / T (reaction time, 3 minutes) = 130 *ΔA / W. The result is shown as following:

The improvement is valid since it was normally produced and reached our expectations--to transfer CotA out of E.coli. Thus, it became one of our favorite parts.

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Parameters
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