Tag
map20 sign

Part:BBa_K2871000:Design

Designed by: Nuttawat Leelahakorn   Group: iGEM18_UCopenhagen   (2018-09-24)
Revision as of 13:57, 9 October 2018 by Nleelahakorn (Talk | contribs) (→‎Design Notes)


Map20, T3SS export signal peptide from Map gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 85
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The amino acid sequence of the signal is obtained from Charpentier & Oswald (2004) paper. The DNA sequence is synthesized by IDT. The glycine-serine linker is added by PCR to decrease folding interaction with a target protein.

Source

Synthetic sequence deduced from Amino acid Sequence from the Enteropathogenic E. coli strain E22. Described in the paper Charpentier & Oswald, 2004.

References

Charpentier & Oswald, 2004. journal of baceteriology. 'Identification of the Secretion and Translocation Domain of the Enteropathogenic and Enterohemorrhagic Escherichia coli Effector Cif, Using TEM-1 β-Lactamase as a New Fluorescence-Based Reporter' DOI: 10.1128/JB.186.16.5486-5495.2004 https://jb.asm.org/content/186/16/5486