Composite

Part:BBa_K2582010

Designed by: Lo Ho Sing   Group: iGEM18_Hong_Kong-CUHK   (2018-10-07)
Revision as of 13:37, 16 October 2018 by Frederickk (Talk | contribs)

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T7 Promoter-RAP(PB2-2247-2268)-T7 Terminator

This part is the T7 RNA Polymerase-based expression cassette of the RNA Aptamer Probe (RAP) BBa_K2582009. It targets the influenza Polymerase Basic 2 (By Marsh et. al. 2008. J. of Virology) by nucleotide 2247-2268.

Usage and Biology

This part is assembled for the expression of a RNA Aptamer Probe, a RNA aptamer that enhances the fluorescence of its docking fluorogen upon binding of a complementary RNA. When transformed into any DE3 strains of E. coli, it can be expressed by IPTG induction.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None