Coding

Part:BBa_K2665011

Designed by: Masahiro Nanbu   Group: iGEM18_Kyoto   (2018-09-25)
Revision as of 10:05, 7 October 2018 by TongYou (Talk | contribs)


TDH3-Mangrin-6xHis-CYC

This part coded the mangrin functional domain(71 amino acids). This part also contains TDH3 promoter, Histag, and CYC1 terminator in order to express constantly in yeasts. We used this part for improving halotorelance of yeast.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 842
    Illegal BamHI site found at 936
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Reference

[1] A. Yamada, T. Saitoh, T. Mimura et al. (2002) Expression of Mangrove Allene Oxide Cyclase Enhances Salt Tolerance in Escherichia coli, Yeast, and Tobacco Cells, Plant and cell physiology 903-910


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Parameters
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