Coding
Part:BBa_K2665011
Designed by: Masahiro Nanbu Group: iGEM18_Kyoto (2018-09-25)
TDH3-Mangrin-6xHis-CYC
Mangrin is derived from mangrove and it is a shaperon like protein and enhances salt tolerance of Escherichia coli, Yeast, and Tobacco Cells. This part coded the functional domain(71 amino acids). This part also contains TDH3 promoter, Histag, and CYC1 terminator in order to express constantly in yeasts. We used this part for improving halotorelance of yeast.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 842
Illegal BamHI site found at 936 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Characterization
For the confirmation of the mangrin function of mangrin, we spotted
Reference
[1] A. Yamada, T. Saitoh, T. Mimura et al. (2002) Expression of Mangrove Allene Oxide Cyclase Enhances Salt Tolerance in Escherichia coli, Yeast, and Tobacco Cells, Plant and cell physiology 903-910
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Categories
Parameters
None |