Coding
TEVp

Part:BBa_K2549013

Designed by: Rongrong Du   Group: iGEM18_Fudan   (2018-10-05)
Revision as of 04:43, 7 October 2018 by Wave (Talk | contribs)


TEV protease

This part is a mutant TEV (tobacco etch virus) protease. The 219 residue of the wild-type TEV protease is mutated from serine to valine to remove autoinactivation and perform a more efficient cleavage[1]. TEV protease is one of the best-characterized enzymes of the viral proteases which have more stringent sequence specificity. We used it to build our IMPLY logic gates. It can also be utilized by other iGEM teams to create their own engineered fusion proteins cleavage system.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 316

Usage and Biology

TBA



References

  1. ↑ Tobacco etch virus protease: mechanism of autolysis and rational design of stable mutants with wild-type catalytic proficiency. Kapust RB, Tözsér J, Fox JD, ..., Copeland TD, Waugh DS. Protein Eng, 2001 Dec;14(12):993-1000 PMID: 11809930
[edit]
Categories
//cds/enzyme/protease
//function/degradation
Parameters
None