Coding
mN1c

Part:BBa_K2549006

Designed by: Rongrong Du   Group: iGEM18_Fudan   (2018-10-02)
Revision as of 08:41, 7 October 2018 by Wave (Talk | contribs) (→‎Usage and Biology)


mouse Notch1 core

This part is the mouse Notch1 minimal regulatory region[1]. It was used as the transmembrane core domain of the SynNotch. However, original reported design has a high background activation in transfected 293T cells. We have put a lot of effect in optimizing this critical transmembrane domain for Notch receptor.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 592


Biology

Significance of Notch signaling
synNotch with α-CD19 against CD19 antigen works extremely well in Morsut L et al 2016

Please refer the original article for more details.

Morsut L at al stated: xxxxxx
Morsut L at al stated: xxxxxx
Morsut L at al stated: xxxxxx
Morsut L at al stated: xxxxxx
Morsut L at al stated: xxxxxx
Morsut L at al stated: xxxxxx
Morsut L at al stated: xxxxxx
Morsut L at al stated: xxxxxx
Morsut L at al stated: xxxxxx
Morsut L at al stated: xxxxxx


References

  1. ↑ Engineering Customized Cell Sensing and Response Behaviors Using Synthetic Notch Receptors. Morsut L, Roybal KT, Xiong X, ..., Thomson M, Lim WA. Cell, 2016 Feb;164(4):780-91 PMID: 26830878; DOI: 10.1016/j.cell.2016.01.012
[edit]
Categories
//cds/membrane
Parameters
None