Coding
LaG17

Part:BBa_K2549004

Designed by: Rongrong Du   Group: iGEM18_Fudan   (2018-10-01)
Revision as of 05:03, 7 October 2018 by Wave (Talk | contribs)


anti-GFP (LaG17)

Anti-GFP (LaG17) is a readily expressible recombinant nanobody which has a relatively low affinity and high specificity against GFP[1]. It was used as the extracellular domain of the SynNotch, thus accomplishing the contact-dependent signal input against GFP. This nanobody recognizes our surEGFP (Part:BBa_K2446051) expressing cells.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 34


Usage and Biology

TBA



References

  1. ↑ A robust pipeline for rapid production of versatile nanobody repertoires. Fridy PC, Li Y, Keegan S, ..., Chait BT, Rout MP. Nat Methods, 2014 Dec;11(12):1253-60 PMID: 25362362; DOI: 10.1038/nmeth.3170
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Categories
//cds
//cds/receptor/antibody
Parameters
protein