Composite

Part:BBa_K2680063

Designed by: Stephanie Do, Xiangyi Fang, Ethan Jones, Jessica Laury, Adam Oliver, Lillian Parr, Chengwu Shen, Tinh Son, Julia Urban, Yashna Verma, Hanmi Zou   Group: iGEM18_William_and_Mary   (2018-10-06)
Revision as of 19:42, 17 October 2018 by Jaurban (Talk | contribs)


39C Temperature-Sensitive mScarlet-I pdt#3b, with mf-lon ssra

Medium-weak 39C Temperature-Sensitive mScarlet-I pdt#3b, with mf-lon ssra. Components: J23107 TPL + ptlpA B0034m mScarlet-I pdt-3b thrL terminator + ptlpAr BCD8 mf-Lon ssrA

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 51
    Illegal NheI site found at 74
    Illegal NotI site found at 613
    Illegal NotI site found at 1920
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 898
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1201
    Illegal AgeI site found at 4275
    Illegal AgeI site found at 4359
    Illegal AgeI site found at 4565
    Illegal AgeI site found at 4590
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None