Composite

Part:BBa_K2680050

Designed by: Stephanie Do, Xiangyi Fang, Ethan Jones, Jessica Laury, Adam Oliver, Lillian Parr, Chengwu Shen, Tinh Son, Julia Urban, Yashna Verma, Hanmi Zou   Group: iGEM18_William_and_Mary   (2018-10-06)
Revision as of 00:01, 18 October 2018 by Ajoliver (Talk | contribs)


J23107 ts-CI+ R0051 mf-Lon

Mechanics

In this circuit, temperature-sensitive CI is expressed by J23107, a medium-weak constitutive promoter. This temperature-sensitive CI then represses the downstream CI promoter. Once the temperature is sufficiently raised, the ts-CI protein denatures, loosing its repression capabilities. Therefore, the CI promoter is freed, allowing for expression of mf-Lon.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 51
    Illegal NheI site found at 74
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2903
    Illegal AgeI site found at 2987
    Illegal AgeI site found at 3193
    Illegal AgeI site found at 3218
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None