Coding
LaG17

Part:BBa_K2549004:Design

Designed by: Rongrong Du   Group: iGEM18_Fudan   (2018-10-01)
Revision as of 11:28, 6 October 2018 by JennyDu (Talk | contribs) (→‎Design Notes)


anti-GFP (LaG17)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 34


Design Notes

LaG17 is served as the extracellular domain of our SynNotch receptor, thus accomplishing contact-dependent signal input against GFP[1].

Source

IDT(gBlock)

References

[1]A robust pipeline for rapid production of versatile nanobody repertoires. Fridy PC, Li Y, Keegan S, ..., Chait BT, Rout MP. Nat Methods, 2014 Dec;11(12):1253-60 PMID: 25362362; DOI: 10.1038/nmeth.3170