Coding

Part:BBa_K2685007:Design

Designed by: LIN, WENXIN   Group: iGEM18_TUST_China   (2018-10-06)
Revision as of 20:18, 17 October 2018 by YuwenZhang (Talk | contribs)


Detecting parts

This part is our part of the test to detect tetracycline in the environment. Because E. coli is not tolerant to tetracycline,

we introduced a resistance mechanism to expand its tolerance to tetracycline, which can be detected at a wider concentration

of tetracycline. The ABS value of E. coli was determined by comparing with that of E.coli. The results showed that the highest

concentration of tetracycline was 10μg/ml in E.coli and 110μg /ml in E.coli.

"T--TUST_China--part_document_072.png"


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1768
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1622
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1068
    Illegal NgoMIV site found at 1228
    Illegal NgoMIV site found at 1596
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 69


Design Notes

detecting tetracycline existing and report by gfp


Source

constructe by us

References