Deleted
Sample Discontinued
Experience: None
Not Used
Get This Part
Plasmid

Part:BBa_K2765019

Designed by: Zhuanzhuan Pu   Group: iGEM18_BIT-China   (2018-10-04)
Revision as of 09:03, 4 October 2018 by Pzz258 (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


Yno1-sgRNA--pSB1C3

This part consists of the promoter SNR52p, sgRNA and the terminator cyc1. The promoter SNR52p is an constitutive promoter that is always used for transcription of small fragment RNA like single guide RNA used in our project. The sgRNA is an artificial RNA that is designed for combination of a target gene. In this project, sgRNA is designed to inhibit expression of yno1, whose overexpression will lead to accumulation of ROS.Here shows the function of sgRNA in this project.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 2029
    Illegal SpeI site found at 2436
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2029
    Illegal NheI site found at 2184
    Illegal SpeI site found at 2436
    Illegal NotI site found at 2035
    Illegal NotI site found at 2443
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2029
    Illegal XhoI site found at 1013
    Illegal XhoI site found at 1905
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 2029
    Illegal SpeI site found at 2436
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 2029
    Illegal XbaI site found at 2044
    Illegal SpeI site found at 2436
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None