Composite

Part:BBa_K2598050:Design

Designed by: Matthew Gao   Group: iGEM18_UCAS-China   (2018-10-02)
Revision as of 11:09, 13 October 2018 by Gaoyuanli1998 (Talk | contribs)

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CGG+T3+PhIF


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 256
    Illegal NgoMIV site found at 265
    Illegal NgoMIV site found at 880
    Illegal NgoMIV site found at 3529
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2595


Design Notes

T3 and PhIF are reversed to avoid leakage. Insulator RiboJ and BydvJ are added to buffer the effect of the genetic context.


Source

The subparts are provided by Christopher A Voigt.

References

Fernandez-Rodriguez, J., Moser, F., Song, M. & Voigt, C. A. Engineering RGB color vision into Escherichia coli. Nature Chemical Biology 13, 706-708 (2017)