Coding

Part:BBa_K2609002:Design

Designed by: Bhaskar Kumawat   Group: iGEM18_IISc-Bangalore   (2018-09-23)
Revision as of 05:34, 2 October 2018 by BhaskarK (Talk | contribs) (→‎References)

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T4 bacteriophage long tail fiber protein gp37 (Modification I)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 424
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was codon optimized for expression in E. coli. The following changes were made to the wild-type protein sequence. ____

A double stop codon is placed at the end to ensure proper termination.

Source

T4 bacteriophage genome, sequence from GenBank: https://www.ncbi.nlm.nih.gov/nuccore/HM137666

References

[1] Leiman, Petr G., et al. "Three-dimensional rearrangement of proteins in the tail of bacteriophage T4 on infection of its host." Cell 118.4 (2004): 419-429.
[2] Yoichi, Masatoshi, et al. "Alteration of tail fiber protein gp38 enables T2 phage to infect Escherichia coli O157: H7." Journal of biotechnology 115.1 (2005): 101-107.
[3] Rakhuba, D. V., et al. "Bacteriophage receptors, mechanisms of phage adsorption and penetration into host cell." Pol. J. Microbiol 59.3 (2010): 145-155.
[4] Bartual, Sergio G., et al. "Structure of the bacteriophage T4 long tail fiber receptor-binding tip." Proceedings of the National Academy of Sciences 107.47 (2010): 20287-20292.