Plasmid
pUC57
Part:BBa_J70004
Designed by: Meagan Lizarazo Group: Knight Lab (2008-01-23)
BioBasic Cloning Plasmid pUC57
Cloning plasmid used by BioBasic for iGEM parts. This plasmid does not have a standard BioBrick cloning site.
Will be adding information once obtained from Justin Pahara (iGEM 07, Alberta). Vector is AmpR.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 396
Illegal XbaI site found at 425
Illegal PstI site found at 453 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 396
Illegal PstI site found at 453 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 396
Illegal BamHI site found at 435 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 396
Illegal XbaI site found at 425
Illegal PstI site found at 453 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 396
Illegal XbaI site found at 425
Illegal PstI site found at 453 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1790
Illegal SapI site found at 707
[edit]
Categories
Parameters
//plasmidbackbone/synthesis
chassis | |
copies | |
mcs | |
origin | |
resistance | A |