Coding
surCD19

Part:BBa_K2549001:Design

Designed by: Rongrong Du   Group: iGEM18_Fudan   (2018-10-01)
Revision as of 09:01, 1 October 2018 by JennyDu (Talk | contribs) (→‎Design Notes)


suface-expressed CD19


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 588
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 757
    Illegal SapI site found at 273
    Illegal SapI.rc site found at 91


Design Notes

Surface-expressed CD19(surCD19) is built by fusing CD8alpha signal peptide and CD19 extracellular domain which has a HA tag on its N terminal and a Myc tag on its C terminal to the transmembrane region of PDGFRbeta from N terminal to C terminal.

Source

from IDT(gBlock)

References

[1]Engineering Customized Cell Sensing and Response Behaviors Using Synthetic Notch Receptors. Morsut L, Roybal KT, Xiong X, ..., Thomson M, Lim WA. Cell, 2016 Feb;164(4):780-91 PMID: 26830878; DOI: 10.1016/j.cell.2016.01.012