DNA

Part:BBa_K2856001:Design

Designed by: Zhuwei Shi   Group: iGEM18_H14Z1_Hangzhou   (2018-09-29)
Revision as of 02:39, 1 October 2018 by Zy007 (Talk | contribs)

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Bifunctional glutamate-cysteine ligase/glutathione synthase (gshF)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sequence has been optimized for expression in Lactococcus lactis. We remove some illegal restriction sites in original squence and use ATG as initiation codon.

Source

The original complete CDS sequence can be obtained from Genebank. Genebank accession: AP018935.1

References