Translational_Unit

Part:BBa_K2705005

Designed by: Danqing Tong   Group: iGEM18_NKU_CHINA   (2018-09-30)
Revision as of 02:30, 1 October 2018 by Tong Danqing (Talk | contribs)


PgltAB-GFP

This part includes a promoter "PgltAB" and GFP, the expression of GFP is repressed by high level cellular glutamate because ProgltAB is upregulated by GltC, which is repressed by cellular glutamate level. A restriction enzyme KpnI cutting site is between PgltAB and GFP. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 671
    Illegal XhoI site found at 572
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None