Part:BBa_K2587024
luxI_luxR_Plux_E
This part is an assembly of different constructs, which then yield a final plasmid, able to self lyse Escherichia coli cells. It is a construct based on the Quorum sensing system, which induces cell lysis upon synthesis of the quorum sensing molecule acyl homoserine lactone. Upon synthesis of this by the LuxI gene, it will bind to the regulator LuxR and then to the promoter pLux, which will activate synthesis of the lysis protein E from the bacteriophage phiX174E.
Usage and Biology This construct can be used to regulate cell density. Compared to the wildtype cells, cells harbouring this plasmid grow slower. Observation of their growth shows a remissive exponential phase, reaching the stationary phase with a lower cell density compared to wild type celly.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 829
Illegal NheI site found at 852 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1082
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 892
Illegal BsaI site found at 1681
Illegal BsaI site found at 1832
Illegal BsaI.rc site found at 1523
Illegal BsaI.rc site found at 1790
None |