Coding

Part:BBa_K2560259:Design

Designed by: Daniel Marchal   Group: iGEM18_Marburg   (2018-09-30)
Revision as of 15:47, 30 September 2018 by Marchal (Talk | contribs) (→‎References)


mcr gene for Malonyl-CoA Reductase from Sulfolobus tokodaii


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 626
    Illegal BglII site found at 819
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part contains the whole coding region from the mcr gene of S. tokodaii (OOOOO) without the startcodon. This region is flanked by overhangs which are Phytobrick- and MoClo-compatible and by two BsaI recognition sites (Weber et al., 2011).

GGTCTCGGATG-coding_region-GCTTTGAGACC


The sequence was codonoptimized for V. natriegens ATCC 14048.


The encoded enzyme catalyzes to conversion of malonyl-CoA into 3-oxopropanoate. By combining this part with BBa_K2560262, it is possible to produce 3-hydroxypropionate from malonyl-CoA.

Source

OOOOO

References

Strauss, G., Fuchs, G., 1993. Enzymes of a novel autotrophic CO2 fixation pathway in the phototrophic bacterium Chloroflexus aurantiacus, the 3-hydroxypropionate cycle. Eur. J. Biochem. 215, 633–43.

Weber, E., Engler, C., Gruetzner, R., Werner, S., Marillonnet, S., 2011. A Modular Cloning System for Standardized Assembly of Multigene Constructs. PLoS One 6, e16765. https://doi.org/10.1371/journal.pone.0016765